Tenacibaculum ascidiaceicola sp nov., isolated from the golden sea squirt Halocynthia aurantium

A Gram-stain-negative, non-flagellated, non-spore-forming bacterial strain motile by gliding, designated RSS1-6(T), was isolated from a golden sea squirt Halocynthia aurantium and its taxonomic position was investigated by using a polyphasic approach. Strain RSS1-6(T) grew optimally at 30-37 degrees C and in the presence of 1.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain RSS1-6(T) fell within the clade comprising species of the genus Tenacibaculum, clustering with the type strains of Tenacibaculum discolor, Tenacibaculum litoreum and Tenacibaculum gallaicum with which it exhibited 16S rRNA gene sequence similarity values of 98.5-99.5 %. Strain RSS1-6(T) contained MK-6 as the predominant menaquinone and iso-C-15 : 0, iso-C-17 : 0 3-OH and summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) as the major fatty acids. The major polar lipids of strain RSS1-6(T) were phosphatidylethanolamine, two unidentified lipids, one unidentified aminophospholipid and one unidentified glycolipid. The DNA G + C content was 32.5 mol% and the mean DNA-DNA relatedness values with the type strains of T. discolor, T. litoreum and T. gallaicum were 17.3-25.2 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RSS1-6(T) is separated from other recognized species of the genus Tenacibaculum. On the basis of the data presented, strain RSS1-6(T) is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum ascidiaceicola sp. nov. is proposed. The type strain is RSS1-6(T) (=KCTC 42702(T) =NBRC 111225(T)).