Journal
JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE
Volume 11, Issue 6, Pages 481-492Publisher
ELSEVIER
DOI: 10.1016/j.jtcme.2021.03.005
Keywords
Antitumor activity; Bioactivity guided structural identification; Mice xenograft model; Nuclear magnetic resonance; Density functional theory; Dynamic light scattering; Scanning electron microscopy
Categories
Funding
- Indian Council of Medical research [59/54/2011/BMS/TRM]
- DBT-SyMeC project [GAP357]
- University Grant Commission [F.15-6 (DEC2013)/2014 (NET), 22/12/2013 (ii) EU-V]
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Moringa oleifera Lam. root extract showed cytotoxic activity, induced apoptosis, and reduced FSHR and c-Myc expression in epithelial ovarian cancer cells. The active compound identified as a naturally hydrated-trifattyglyceride has a folded amphipathic structure for easy transportation in biological systems.
Background and aim: Epithelial ovarian cancer has the deadliest prognosis amongst gynaecological cancers, warranting an unmet need for newer drug targets. Based on its anticancer as well as abortifacient potential, Moringa oleifera Lam. root was hypothesized to have some implications in follicle stimulating hormone receptor (FSHR) dependent cancers like epithelial ovarian cancer. Experimental procedure: Effect of Moringa oleifera Lam. root extract (MRE) was studied in epithelial ovarian cancer cell line through in vitro studies viz. MTT assay, clonogenic assay, cell cycle analysis, flow cytometry, western blot analysis, immunocytochemical analysis of FSHRand c-Myc expression and in vivo studies viz. effect of MRE in mice model of ovarian carcinoma. The structure of the active compound of MRE was elucidated following solvent extraction, purification through column chromatography, preparative TLC and bioactivity guided structural identification through 1H-NMR, 13C-NMR, DEPT135, ESIMS,FT-IR spectrophotometry, UV-vis-NIR spectrophotometry and DFT study. Results and conclusion: Crude MRE displayed cytotoxic activity, induced apoptosis, and attenuated expression of FSHR and c-Myc in ovarian cancer cell line OAW42. MRE also attenuated expression of CD31, FSHR, and c-Myc in tumour xenograft mouse model. Finally, the active compound purified from ethyl acetate-n-hexane subfraction of MRE, that attenuated viability of ovarian carcinoma cell lines and reduced FSHR and c-Myc expression, was identified as a naturally hydrated-trifattyglyceride, showing aDFT-optimized folded amphipathic structure for easy transportation through hydrophilic and hydrophobic regions in a biological system, indicating its immense therapeutic relevance in epithelial ovarian carcinoma. (c) 2021 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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