Journal
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
Volume 66, Issue 2, Pages 82-88Publisher
TAYLOR & FRANCIS AS
DOI: 10.1080/00914037.2016.1182915
Keywords
3DOM; binding properties; BSA; colloidal crystal; molecular imprinting
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Funding
- National Natural Science Foundation of China [81303199]
- Distinguished Young Talents in Higher Education of Guangdong, China [K5090006]
- Youth Elite Project of GUCM [AFD015141Z0216]
- Science Program for Overseas Scholar of Guangzhou University of Chinese Medicine [AFD015141Z0230]
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A novel protein molecularly imprinted microsphere with three-dimensionally ordered macroporous (3DOM) structure has been successfully prepared with a combination of colloidal crystal template method and molecular imprinting technique by using bovine serum albumin as template, acrylamide as functional monomer, and N,N-methylenebisacrylamide as crosslinker, respectively. SEM shows that the polymeric microspheres have a highly ordered three-dimensional interconnected macroporous structure with typical face-centered cubic lattice. The isothermal and kinetic adsorption behaviors of the polymer were investigated. Compared with the traditional bulk polymer, the 3DOM imprinted microspheres show higher binding rate constant, adsorption capability and specificity to template molecules.
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