Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 504, Issue 1-2, Pages 59-69Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2016.03.013
Keywords
Mucosal immunization; Vaccine adjuvants; Antigen delivery; Poly-epsilon-caprolactone (PCL)/chitosan nanoparticles; Dose-response; Polymeric biomaterials
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Funding
- FEDER funds through the Operational Programme Competitiveness Factors - COMPETE
- FCT - Foundation for Science and Technology [PTDC/SAU-FAR/11504, DFRH - SFRH/BD/81350/2011, UID/NEU/04539/20134/2009]
- FCT [REDE/1510/RME/2005]
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Among new strategies to increase hepatitis B virus (HBV) vaccination, especially in developing countries, the development of self-administered vaccines is considered one of the most valuable. Nasal vaccination using polymeric nanoparticles (NPs) constitutes a valid approach to this issue. In detail, poly-epsilon-caprolactone (PCL)/chitosan NPs present advantages as a mucosal vaccine delivery system: the high resistance of PCL against degradation in biological fluids and the mucoadhesive and immunostimulatory properties of chitosan. In vitro studies revealed these NPs were retained in a mucus-secreting pulmonary epithelial cell line and were capable of entering into differentiated epithelial cells. The intranasal (IN) administration of 3 different doses of HBsAg (1.5 mu g, 5 mu g and 10 mu g) adsorbed on a fixed amount of PCL/chitosan NPs (1614 mu g) generated identical titers of serum anti-HBsAg IgG and anti-HBsAg sIgA in mice nasal secretions. Besides other factors, the NP surface characteristics, particularly, zeta potential differences among the administered formulations are believed to be implicated in the outcome of the immune response generated. (C) 2016 Elsevier B.V. All rights reserved.
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