LVSEP Analysis of Phosphopeptides in Dynamically PVP-Coated Capillaries and Microchannels

To achieve highly sensitive analyses of phosphopeptides with simple experimental procedures in capillary electrophoresis (CE) and microchip electrophoresis (MCE), large-volume sample stacking with an electroosmotic flow pump (LVSEP) was performed in dynamically poly(vinyl pyrrolidone) (PVP)-coated capillaries to suppress the electroosmotic flow. In the analysis of monophosphopeptide (MPP) from bovine beta-casein, good enrichments were attained with the sensitive enhancement factor (SEF) of 260 and 400 in CE and MCE, respectively, by using 0.2% PVP in 20 mM HEPES buffer (pH 9.0) in LVSEP.

Automated summary

In this study, highly sensitive analyses of phosphopeptides were achieved in capillary electrophoresis (CE) and microchip electrophoresis (MCE) by using large-volume sample stacking with an electroosmotic flow pump (LVSEP) in dynamically poly(vinyl pyrrolidone) (PVP)-coated capillaries to suppress the electroosmotic flow. Good enrichments with sensitive enhancement factors (SEF) of 260 and 400 were obtained in CE and MCE, respectively, by using 0.2% PVP in 20 mM HEPES buffer (pH 9.0) in LVSEP.