Journal
INTERNATIONAL JOURNAL OF ONCOLOGY
Volume 49, Issue 6, Pages 2294-2302Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2016.3727
Keywords
kahweol; non-small cell lung cancer; basic transcription; factor 3; ERK; apoptosis
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Funding
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ01116401]
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Kahweol, a diterpene molecule, has antiproliferative effects on several types of human cancer cells, but whether it has apoptotic effect in non-small cell lung cancer (NSCLC) is not known. To explore this possibility, we incubated cells from two NSCLC cell lines, NCI-H358 and NCI-H1299, with different concentrations of kahweol and used the MTS assay, DAPI staining, propidium iodide staining, Annexin V staining, immunocytochemical test, and western blot analysis to characterize this molecule and the signaling pathway underlying its effects. The kahweol-treated cells showed significantly decreased cell viability, increased nuclear condensation, and an increased number of Annexin V-positive NSCLC cells. Suppression of basic transcription factor 3 (BTF3) was followed by apoptosis induced by kahweol via the ERK-mediated signaling pathway in a dose- and time-dependent manner. In addition, kahweol modulated the protein expression of BTF3 genes involved in cell-cycle regulation and apoptosis-related proteins, resulting in apoptotic cell death. Our results collectively indicated that kahweol inhibited the proliferation of NSCLC cells through ERK-mediated signaling pathways and the downregulation of BTF3.
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