Journal
MOLECULAR AND CLINICAL ONCOLOGY
Volume 16, Issue 1, Pages -Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mco.2021.2452
Keywords
extracellular microRNA; radiotherapy; colorectal cancer; micronuclei
Categories
Funding
- KAKENHI
- Fund for the Promotion of Joint International Research (Fostering Joint International Research) [17KK0181, 19K22731]
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Changes in extracellular microRNAs may serve as biomarkers for radiation-induced damage to human colorectal cancer cells. Specific microRNAs showed a significantly positive correlation with micronuclei frequency in a dose-dependent manner. These findings suggest that these specific microRNAs could be potential markers for cell damage and radiotherapy response in colorectal cancer, but further validation is needed in serum samples from patients undergoing radiotherapy.
Biomarkers of tumour response to radiotherapy may help optimise cancer treatment. The aim of the present study was to identify changes in extracellular microRNAs (miRNAs) as a biomarker of radiation-induced damage to human colorectal cancer cells. HCT116 cells were exposed to increasing doses of X-rays, and extracellular miRNAs were analysed by microarray. The results were correlated with the frequency of micronuclei. A total of 59 miRNAs with a positive correlation and 4 with a negative correlation between dose (up to 6 Gy) and extracellular miRNA expression were identified. In addition, for doses between 0 and 10 Gy, 12 miRNAs among those 59 miRNAs with a positive correlation were identified; for these extracellular miRNAs, a significantly positive correlation was observed between their expression and the frequency of micronuclei for doses up to 10 Gy. These results suggest that specific miRNAs may be considered as cell damage markers and may serve as secreted radiotherapy response biomarkers for colorectal cancer; however, the results must be further validated in serum samples collected from patients undergoing radiotherapy.
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