Journal
VETERINARY WORLD
Volume 14, Issue 10, Pages 2634-2639Publisher
VETERINARY WORLD
DOI: 10.14202/vetworld.2021.2634-2639
Keywords
chickens; cytochrome c oxidase subunit I gene; leucocytozoonosis; megaloschizonts
Funding
- Research Fund of the Faculty of Veterinary Medicine, Khon Kaen University, Thailand
- Faculty of Veterinary Medicine, Khon Kaen University, Thailand [KKU Vet. Res. VM008/2564]
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This study aimed to characterize Leucocytozoon caulleryi from backyard chickens in Khon Kaen Province, Thailand. Through necropsy and molecular analysis, it was determined that L. caulleryi was the causal agent of leucocytozoonosis and closely related to strains reported in Japan.
Aim: The aim of this study was to characterize Leucocytozoon caulleryi from backyard chickens in Khon Kaen Province, Thailand. Materials and Methods: Tissue samples were collected from backyard chickens suspected to have leucocytozoonosis and subjected to histopathology examination. The BLAST Tool at NCBI GenBank (Basic Local Alignment Research Tools) (http://www.ncbi.nlm.nih.gov/BLAST) was used to identify the nucleotide sequence of the partial cytochrome c oxidase subunit I (cox I) gene. A Phylogenetic tree for analysis of L. caulleryi was constructed by using MEGA 7.0 software (https:// www.megasoftware.net/). Results: The necropsy results revealed the subcutaneous hemorrhages of pectoral muscles, multifocal hemorrhages of the thymus and pectoral muscles, hemorrhage of the proventriculus and peritoneal cavity, and megaloschizonts of the pancreas and intestine, including subcapsular hemorrhages of the liver. Microscopic examination revealed numerous megaloschizonts of Leucocytozoon spp. in the pectoral muscles, intestine, pancreas, and thymus. Molecular analysis of the partial coxI gene showed that the causal agent was closely related to L. caulleryi reported in Japan. Conclusion: From these results, L. caulleryi was determined to be the causal agent of leucocytozoonosis and was closely associated with L. caulleryi reported in Japan.
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