Journal
DATA IN BRIEF
Volume 40, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.dib.2021.107687
Keywords
Regulatory T cell; Conventional T cell; Proteomics; Tandem mass spectrometry; LC-MS/MS
Categories
Funding
- International Postgraduate Research Scholarship, The University of Queensland, Brisbane, Australia
- National Health and Medical Research Council Career Development Level 2 Fellowship [1131732]
- PhD Top-up Scholarship (QIMRB)
Ask authors/readers for more resources
Regulatory T cells (Tregs) are crucial for maintaining immune tolerance and homeostasis. A comprehensive proteomic dataset of Tregs from healthy individuals has been generated, which identifies specific biological processes and pathways associated with Tregs. This dataset provides valuable insights into Treg immunophysiology and may lead to the discovery of novel therapeutics for Treg regulation.
Regulatory T cells (Tregs) play a core role in maintaining immune tolerance, homeostasis, and host health. High-resolution analysis of the Treg proteome is required to identify enriched biological processes and pathways distinct to this important immune cell lineage. We present a comprehensive proteomic dataset of Tregs paired with conventional CD4(+) (Conv CD4(+)) T cells in healthy individuals. Tregs and Conv CD4(+) T cells were sorted to high purity using dual magnetic bead-based and flow cytometry-based methodologies. Proteins were trypsin-digested and analysed using label-free data-dependent acquisition mass spectrometry (DDA-MS) followed by label free quantitation (LFQ) proteomics analysis using MaxQuant software. Approximately 4,000 T cell proteins were identified with a 1% false discovery rate, of which approximately 2,800 proteins were consistently identified and quantified in all the samples. Finally, flow cytometry with a monoclonal antibody was used to validate the elevated abundance of the protein phosphatase CD148 in Tregs. This proteomic dataset serves as a reference point for future mechanistic and clinical T cell immunology and identifies receptors, processes, and pathways distinct to Tregs. Collectively, these data will lead to a better understanding of Treg immunophysiology and potentially reveal novel leads for therapeutics seeking Treg regulation. (c) 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available