Acetylcholinesterase Immobilized on Magnetic Mesoporous Silica Nanoparticles Coupled with Fluorescence Analysis for Rapid Detection of Carbamate Pesticides

Herein, we successfully synthesized a magnetic mesoporous material, Fe3O4@SiO2@mSiO(2), and acetylcholinesterase (AChE) was immobilized by a covalent bonding method. The tolerance ability of immobilized AChE (by magnetic mesoporous silica nanoparticles) in different organic solvents was better than that of free AChE. Furthermore, the magnetic character of the fixed AChE improved the detection sensitivity and reliability for carbofuran, methomyl, isoprocarb, and carbaryl. The limit of detection (LOD) of carbofuran, methomyl, isoprocarb, and carbaryl was 0.01, 0.22, 0.26, and 0.43 mu M, respectively. It shows a good correlation between carbamate pesticides (CMs) and the inhibition rate of Fe3O4@SiO2@mSiO(2)-AChE in a given concentration range. The linear ranges of carbofuran, methomyl, isoprocarb, and carbaryl were 0.02-0.90, 0.31-12.33, 0.27-5.17, and 0.50-14.91 mu M, respectively. Chinese cabbage and cucumber spiked with four CMs were used as field-incurred samples to prove the feasibility of the developed method. According to the maximum residue limits, the results were satisfactory, with recovery rates between 77.03 and 110.07%, and the RSDs ranged from 3.17 to 6.79%. The stability of immobilized AChE in organic solvents was improved with enhanced sensitivity for detecting pesticide residues.

Automated summary

In this study, a magnetic mesoporous material was successfully synthesized and acetylcholinesterase (AChE) was immobilized on it. The magnetic properties of the immobilized AChE improved the detection sensitivity and reliability for pesticide residues.