Journal
LIFE SCIENCE ALLIANCE
Volume 5, Issue 3, Pages -Publisher
LIFE SCIENCE ALLIANCE LLC
DOI: 10.26508/lsa.202101285
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Funding
- Emory University School of Medicine Flow Cytometry Core
- National Institutes of Health/National Institute of Allergy and Infectious Diseases [1R01AI121252, 1P01AI125180, U01AI141993, U54CA260563, U19AI110483, R37AI049660, T32AI070081]
- Bill & Melinda Gates Foundation [INV-002351]
- Emory and Pediatric Flow Cytometry Core (ECFCC)
- Bill and Melinda Gates Foundation [INV-002351] Funding Source: Bill and Melinda Gates Foundation
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This study reveals the mechanisms by which circulating antibody secreting cells (ASCs) transform into long-lived plasma cells (LLPCs). LLPCs in the bone marrow (BM) have distinct morphological, transcriptomic, and epigenetic features compared to blood ASCs. LLPCs up-regulate prosurvival genes while simultaneously downregulating pro-apoptotic genes. The study also demonstrates that blood ASCs can undergo similar morphological and molecular changes by mimicking the BM environment in vitro.
Antibody secreting cells (ASCs) circulate after vaccination and infection and migrate to the BM where a subset known as longlived plasma cells (LLPCs) persists and secrete antibodies for a lifetime. The mechanisms by which circulating ASCs become LLPCs are not well elucidated. Here, we show that human blood ASCs have distinct morphology, transcriptomes, and epigenetics compared with BM LLPCs. Compared with blood ASCs, BM LLPCs have decreased nucleus/cytoplasm ratio but increased endoplasmic reticulum and numbers of mitochondria. LLPCs up-regulate prosurvival genes MCL1, BCL2, and BCL-XL while simultaneously downregulating pro-apoptotic genes HRK1, CASP3, and CASP8. Consistent with reduced gene expression, the pro-apoptotic gene loci are less accessible in LLPCs. Of the pro-survival genes, only BCL2 is concordant in gene up-regulation and loci accessibility. Using a novel in vitro human BM mimetic, we show that blood ASCs undergo similar morphological and molecular changes that resemble ex vivo BM LLPCs. Overall, our study demonstrates that early-minted blood ASCs in the BM microniche must undergo morphological, transcriptional, and epigenetic changes to mature into apoptotic-resistant LLPCs.
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