Cytogenetic effect of some nanostructure polymers prepared via gamma irradiation on Vicia faba plant

Background: Nourishment plants during the field time is a must; to have healthy, high productive and self-propagating plants. The trendy nano-fertilizers came to the front in modernized agriculture seeking for minimizing the soil suffocation with other chemical fertilizers in the bulk size. Nano-fertilizers may represent a way out of shot as they are completely absorbed by plant due to their small size, also it magnifies the benefit to the plant due to its high surface area. Nano-fertilizers are introduced via different way of synthesis methods. In this work, three of new nanocomposites are prepared in nano form via Gamma irradiation from Cobalt 60 source at irradiation dose 5 KGy.These composites which can supply plants with P, Zn elements needs to be revised for their safety usage in agriculture. Methodology: Three compounds; Zinc oxide, phosphorous and the mixed Zinc-phosphorous elements were prepared in nano-composite forms coated with PVP as a shell and then characterized by HR-TEM, UV and FT-IR to emphasize their new sizes and shapes, then, they were examined for their cytotoxicity in three concentrations (0.5, 1 and 2%) on Vicia faba plants; after 3 h of direct roots treatment. Cytotoxicity test concerned the mitotic index, phase index, abnormal mitosis and the type of the aberrations at each phase. Results: The three tested NPs exerted mito-accelerating effect on root meristematic cells. However, concentration-dependent genotoxicity was also an evident. Conclusion: The three examined nano-composites may recommend to be used in the lowest examined concentrations to minimize its harm effect on the plant cell and keep their benefits to the environment. It also recommended to count the Zn/P mix NPs over ZN or P separately as it induces an intermediating cytogenetic effect on mitosis apparatus of Vicia faba plant.

Automated summary

In modernized agriculture, nano-fertilizers have been introduced as an alternative to reduce soil suffocation. In this study, three new nanocomposites were prepared and their cytotoxicity on plant cells were evaluated. The results showed that these nanocomposites promoted mitosis in root cells, but also exhibited concentration-dependent genotoxicity. Therefore, it is recommended to use these nanocomposites at the lowest concentration to minimize harm on plant cells.