Journal
BIOSENSORS-BASEL
Volume 12, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/bios12020053
Keywords
RNA sensing; CRISPR-Cas; spacer; in vitro; PAM; sensor design
Funding
- King Abdullah University of Science and Technology (KAUST) through the baseline fund [REI/1/4204-01]
- Office of Sponsored Research (OSR)
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CRISPR-Cas systems have great potential for RNA biosensing, but there is currently no systematic guide for selecting the most appropriate RNA-targeting CRISPR-Cas system. This study proposes a set of systematic selection criteria and identifies four candidates for in vitro RNA.
CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a given application among thousands of potential candidates. We provide an overview of the currently described Cas effector systems and review existing Cas-based RNA detection methods. We then propose a set of systematic selection criteria for selecting CRISPR-Cas candidates for new applications. Using this approach, we identify four candidates for in vitro RNA.
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