Journal
COMMUNICATIONS BIOLOGY
Volume 4, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s42003-021-02596-y
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Funding
- Chao Family Comprehensive Cancer Center Experimental Tissue Resource
- National Cancer Institute of the National Institutes of Health [P30CA062203]
- National Cancer Institute [K22 CA190511, 1R01CA234496, 4R00CA181490]
- American Cancer Society [134389-RSG-20-039-01-DDC, 132551-RSG-18-194-01-DDC]
- Chan-Zuckerberg Initiative [CZF2019-002432]
- National Institutes of Health [P41-GM103540, T32CA009054]
- National Science Foundation [1847005, GRFP DGE-1839285]
- V Foundation [V2019-019]
- University of Hail, Hail, Saudi Arabia
- Canadian Institutes of Health Research Postdoctoral Fellowship
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1847005] Funding Source: National Science Foundation
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A 3D culture-transplant system was developed for studying cancer metastasis using patient-derived xenograft (PDX) tumor cells, showing that OXPHOS inhibition attenuates lung metastatic capacity of breast cancer cells and that overexpression of the metabolic enzyme NME1 increases lung metastasis. The system allows for lentiviral engineering and dissemination of tumor cells, validating its authenticity for investigations of metastasis and revealing a new role for NME1 in promoting breast cancer metastasis.
Ma, Hernandez et al develop a 3D culture-transplant system to enable studies of cancer metastasis using patient-derived xenograft (PDX) tumor cells. Using this system, they find that OXPHOS inhibition attenuates the lung metastatic capacity of breast cancer cells and that overexpression of the metabolic enzyme NME1 increases lung metastasis. Metastasis is a fatal disease where research progress has been hindered by a lack of authentic experimental models. Here, we develop a 3D tumor sphere culture-transplant system that facilitates the growth and engineering of patient-derived xenograft (PDX) tumor cells for functional metastasis assays in vivo. Orthotopic transplantation and RNA sequencing (RNA-seq) analyses show that PDX tumor spheres maintain tumorigenic potential, and the molecular marker and global transcriptome signatures of native tumor cells. Tumor spheres display robust capacity for lentiviral engineering and dissemination in spontaneous and experimental metastasis assays in vivo. Inhibition of pathways previously reported to attenuate metastasis also inhibit metastasis after sphere culture, validating our approach for authentic investigations of metastasis. Finally, we demonstrate a new role for the metabolic enzyme NME1 in promoting breast cancer metastasis, providing proof-of-principle that our culture-transplant system can be used for authentic propagation and engineering of patient tumor cells for functional studies of metastasis.
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