Journal
ISCIENCE
Volume 24, Issue 10, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.isci.2021.103179
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Funding
- German Research Council (Deutsche Forschungsgemeinschaft) [Gi 540-3/2, INST 409/223-1 FUGG]
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EpCAM is expressed in various tissues and cells, with interactions identified with regulators of endosomal trafficking and membrane recycling. Endocytosis and membrane recycling play a role in regulating EpCAM levels during differentiation and EMT induction in ESCs and carcinoma cells.
Transmembrane epithelial cell adhesion molecule (EpCAM) is expressed in epithelia, carcinoma, teratoma, and embryonic stem cells (ESCs). EpCAM displays spatiotemporal patterning during embryogenesis, tissue morphogenesis, cell differentiation, and epithelial-to-mesenchymal transition (EMT) in carcinomas. Potential interactors of EpCAM were identified in murine F9 teratoma cells using a stable isotope labeling with amino acids in cell culture-based proteomic approach (n = 77, enrichment factor >3, p value <= 0.05). Kyoto Encyclopedia of Genes and Genomes and gene ontology terms revealed interactions with regulators of endosomal trafficking and membrane recycling, which were further validated for Rab5, Rab7, and Rab11. Endocytosis and membrane recycling of EpCAM were confirmed in mF9 cells, E14TG2 alpha ESC, and Kyse30 carcinoma cells. Reduction of EpCAM during mesodermal differentiation and TGF beta-induced EMT correlated with enhanced endocytosis and block or reduction of recycling in ESCs and esophageal carcinoma cells. Hence, endocytosis and membrane recycling are means of regulation of EpCAM protein levels during differentiation of ESC and EMT induction in carcinoma cells.
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