Journal
ISCIENCE
Volume 25, Issue 2, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.isci.2022.103754
Keywords
'symbiotic' calcium-; moss homologs
Categories
Funding
- NSF [MCB-0723931, ISO-1339239]
- Vilas Faculty Young/Mid-Career Award
- NIH [5T32GM007133-40, DGE-1106400]
- NSF Graduate Research Fellowship Program [DGE-1256259]
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This study reveals that the CCaMK-IPD3 signaling pathway in the moss Physcomitrium plays a functional role in stress-associated developmental reprogramming.
Symbioses between angiosperms and rhizobia or arbuscular mycorrhizal fungi are controlled through a conserved signaling pathway. Microbe-derived, chitin based elicitors activate plant cell surface receptors and trigger nuclear calcium oscillations, which are decoded by a calcium/calmodulin-dependent protein kinase (CCaMK) and its target transcription factor interacting protein of DMI3 (IPD3). Genes encoding CCaMK and IPD3 have been lost in multiple non-mycorrhizal plant lineages yet retained among non-mycorrhizal mosses. Here, we demonstrated that the moss Physcomitrium is equipped with a bona fide CCaMK that can functionally complement a Medicago loss-of-function mutant. Conservation of regulatory phosphosites allowed us to generate predicted hyperactive forms of Physcomitrium CCaMK and IPD3. Overexpression of synthetically activated CCaMK or IPD3 in Physcomitrium led to abscisic acid (ABA) accumulation and ectopic development of brood cells, which are asexual propagules that facilitate escape from local abiotic stresses. We therefore propose a functional role for Physcomitrium CCaMK-IPD3 in stress-associated developmental reprogramming
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