Journal
JOURNAL OF FUNGI
Volume 8, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/jof8020160
Keywords
carotenogenic yeast; Plackett-Burman; central composite design
Categories
Funding
- Taif University Researchers Supporting Project, Taif University, Taif, Saudi Arabia [TURSP-2020/307]
Ask authors/readers for more resources
Rhodotorula yeasts, known for their ability to produce carotenoids, have great industrial value. In particular, the yeast strain Rhodotorula sp. (ATL72) has been found to be a promising producer of high concentrations of carotenoids. By optimizing the production medium and cultivation conditions, the carotenoid production can be significantly improved.
Rhodotorula yeasts which are known as carotenogenic yeasts have a great industrial value due to their ability to produce carotenoids. In particular, the isolated yeast Rhodotorula sp. (strain ATL72) has been reported to be a promising producer of high concentrations of carotenoids. A combination of central composite design (CCD) and Plackett-Burman (PB) design was used to optimize carotenoids produced by this yeast. The optimum production of carotenoids was completed when the yeast was grown in a production medium composed of 3.7 g/L malt extract, 7.7 g/L fructose, 9 g/L urea, 35 g/L NaCl, and 1 g/L yeast extract at 27.5 degrees C, pH 6.7, and 180 rpm. Two batch runs in 1 L and 7 L bioreactors were conducted which increased the productivity of carotenoid concentration from 21.5 mg/L after 98 h of incubation at the level of the shake flask to 229.9 mg/L after 47 h of incubation at the level of 7 L bioreactor. The carotenoid pigment was extracted in dimethylsulfoxide (DMSO), acetone, petroleum ether, and sodium chloride, and subsequently identified and characterized using UV-visible scanning, thin layer chromatography, and gas chromatography/mass spectrometry.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available