Journal
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
Volume 13, Issue 4, Pages 977-999Publisher
ELSEVIER INC
DOI: 10.1016/j.jcmgh.2021.12.012
Keywords
Microglia; P2RY12; TRPV1 Neurons; Visceral Pain
Categories
Funding
- Crohns and Colitis Canada
- Canadian Institutes of Health Research
- Alberta Childrens Hospital Research Institute
- Eyes High fellowship initiative at the University of Calgary
- Canadian Association of Gastroenterology (CAG)
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The research explores the mechanisms of chronic abdominal pain in IBD patients, focusing on the role of neuronal activity in microglial activation and visceral hypersensitivity. It suggests that targeting microglial P2Y12 signaling could potentially alleviate pain in patients in remission.
BACKGROUND & AIMS: Chronic abdominal pain is a common symptom of inflammatory bowel diseases (IBDs). Peripheral and central mechanisms contribute to the transition from acute to chronic pain during active disease and clinical remission. Lower mechanical threshold and hyperexcitability of visceral afferents induce gliosis in central pain circuits, leading to persistent visceral hypersensitivity (VHS). In the spinal cord, microglia, the immune sentinels of the central nervous system, undergo activation in multiple models of VHS. Here, we investigated the mechanisms of microglia activation to identify centrally acting analgesics for chronic IBD pain.& nbsp;METHODS: Using Designer Receptors Exclusively Activated by Designer Drugs (DREADD) expressed in transient receptor potential vanilloid member 1-expressing visceral neurons that sense colonic inflammation, we tested whether neuronal activity was indispensable to control microglia activation and VHS. We then investigated the neuron-microglia signaling sys-tem involved in visceral pain chronification.& nbsp;RESULTS: We found that chemogenetic inhibition of transient receptor potential vanilloid member 1 thorn visceral afferents prevents microglial activation in the spinal cord and subsequent VHS in colitis mice. In contrast, chemogenetic activation, in the absence of colitis, enhanced microglial activation associated with VHS. We identified a purinergic signaling mechanism mediated by neuronal adenosine triphosphate (ATP) and microglial P2Y12 receptor, triggering VHS in colitis. Inhibition of P2RY12 prevented microglial reactivity and chronic VHS post-colitis.& nbsp;CONCLUSIONS: Overall, these data provide novel insights into the central mechanisms of chronic visceral pain and suggest that targeting microglial P2RY12 signaling could be harnessed to relieve pain in patients with IBD who are in remission.& nbsp;
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