Journal
BIOACTIVE MATERIALS
Volume 14, Issue -, Pages 350-363Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2022.01.040
Keywords
Single cell RNA-Seq; Annulus fibrosus-derived stem cells; Lineage tracing; Composite hydrogel; Annulus fibrosus reconstruction
Funding
- State Key Program of National Natural Science Foundation of China [81730065, 82130070]
- Projects of Interna-tional Cooperation and Exchanges ofNational Natural Science Founda-tion of China [82020108019]
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This study decoded the mouse AF cell atlas, identified a new population of AF cells, and proposed a novel strategy for AF reconstruction based on composite hydrogels, demonstrating the potential for successful reconstruction of AF defects.
Low back pain is one of the most serious public health problems worldwide and the major clinical manifestation of intervertebral disc degeneration (IVDD). The key pathological change during IVDD is dysfunction of the annulus fibrosus (AF). However, due to the lack of an in-depth understanding of AF biology, the methods to reconstruct the AF are very limited. In this study, the mice AF cell atlas were decoded by single-cell RNA sequencing to provide a guide for AF reconstruction. The results first identify a new population of AF cells, fibrochondrocyte-like AF cells, which synthesize both collagen I and collagen II and are potential functional cells for AF reconstruction. According to the dual features of the AF extracellular matrix, a composite hydrogel based on the acylation of methacrylated silk fibroin with methacrylated hyaluronic acid was produced. To obtain the ability to stimulate differentiation, the composite hydrogels were combined with a fibrochondmcyte-inducing supplement. Finally, reconstruction of the AF defects, by the novel AF stem cell-loaded composite hydrogel, could be observed, its amount of chondroid matrices recovered to 31.7% of AF aera which is significantly higher than that in other control groups. In summary, this study decodes the AF cell atlas, based on which a novel strategy for AF reconstruction is proposed.
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