Journal
BIOACTIVE MATERIALS
Volume 6, Issue 11, Pages 3987-3998Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2021.03.029
Keywords
Chitosan microcarriers; Polylysine; Adipose-derived stem cells; Peripheral nerve repair; Cell transplantation
Funding
- National Natural Science Foundation of China [51773018, 51973018]
- Key Research and Development Projects of People's Liberation Army [BWS17J036]
Ask authors/readers for more resources
Cell transplantation using microcarriers is an effective strategy to improve nerve regeneration and repair outcomes, presenting a new therapeutic approach for peripheral nerve repair.
Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits (NGCs). However, problems such as low loading efficiency and cell anoikis undermine the outcomes. Microcarriers are efficient 3D cell culture scaffolds, which can also prevent cell anoikis by providing substrate for adhesion during transplantation. Here, we demonstrate for the first time microcarrier-based cell transplantation in peripheral nerve repair. We first prepared macroporous chitosan microcarriers (CSMCs) by the emulsion-phase separation method, and then decorated the CSMCs with polylysine (pl-CSMCs) to improve cell affinity. We then loaded the pl-CSMCs with adipose-derived stem cells (ADSCs) and injected them into electrospun polycaprolactone/chitosan NGCs to repair rat sciatic nerve defects. The ADSCs-laden pl-CSMCs effectively improved nerve regeneration as demonstrated by evaluation of histology, motor function recovery, electrophysiology, and gastrocnemius recovery. With efficient cell transplantation, convenient operation, and the multiple merits of ADSCs, the ADSCsladen pl-CSMCs hold good potential in peripheral nerve repair.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available