Journal
SMALL METHODS
Volume 6, Issue 2, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smtd.202100912
Keywords
biomolecular electron controllers; biomoletrons; cell differentiation; cell therapy; regenerative medicine; spatiotemporal differentiation control
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [2019R1A2C3002300]
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT [NRF-2021M3H4A1A01079399]
- Samsung Research Center Funding for Future Research
- National Research Foundation of Korea [2021M3H4A1A01079399] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The study introduces a novel cell differentiation biomolecular electron controller, achieving successful spatiotemporal control of SH-SY5Y cell differentiation, providing a promising strategy for regenerative medicine and cell therapy.
In vitro spatiotemporal control of cell differentiation is a critical issue in several biomedical fields such as stem cell therapy and regenerative medicine, as it enables the generation of heterogeneous tissue structures similar to those of their native counterparts. However, the simultaneous control of both spatial and temporal cell differentiation poses important challenges, and therefore no previous studies have achieved this goal. Here, the authors develop a cell differentiation biomolecular electron controller (Biomoletron) composed of recombinant proteins, DNA, Au nanoparticles, peptides, and an electrically released complex with retinoic acid (RA) to spatiotemporally control SH-SY5Y cell differentiation. RA is only released from the Biomoletron when the complex is electrically stimulated, thus demonstrating the temporal control of SH-SY5Y cell differentiation. Furthermore, by introducing a patterned Au substrate that allows controlling the area where the Biomoletron is immobilized, spatiotemporal differentiation of the SH-SY5Y cell is successfully achieved. Therefore, the proposed Biomoletron-mediated differentiation method provides a promising strategy for spatiotemporal cell differentiation control with applications in regenerative medicine and cell therapy.
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