A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge

Article Biotechnology & Applied Microbiology

Fiber1, but not fiber2, is the essential fiber gene for fowl adenovirus 4 (FAdV-4)

Xiaohui Zou et al.

Summary: Briefly, the study found that fiber1 is crucial for the attachment of FAdV-4 virus and is essential, while fiber2, although contributing significantly to virus virulence, is dispensable.

JOURNAL OF GENERAL VIROLOGY (2021)

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Article Biotechnology & Applied Microbiology

Fiber modifications enable fowl adenovirus 4 vectors to transduce human cells

Wenfeng Zhang et al.

Summary: By modifying the fibers of FAdV-4 vectors, the gene transduction efficiency to human cells can be significantly enhanced. Specifically, inserting exogenous peptides into the fiber knob proteins can promote the transduction of the vectors to different types of human cells.

JOURNAL OF GENE MEDICINE (2021)

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Article Veterinary Sciences

Experimental co-infection of variant infectious bursal disease virus and fowl adenovirus serotype 4 increases mortality and reduces immune response in chickens

A-Hui Xu et al.

Summary: Co-infection of variant IBDV ZD-2018-1 and FAdV-4 HB1501 significantly enhances the pathogenicity of FAdV-4 and reduces the immune response in chickens, with delayed expression of certain cytokines and lower antibody response levels observed in coinfected chickens compared to those infected with FAdV-4 alone. These findings lay the groundwork for further exploration of the interaction mechanism in IBDV and FAdV-4 co-infection.

VETERINARY RESEARCH (2021)

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Article Veterinary Sciences

A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus

Quan Xie et al.

Summary: This study developed a recombinant virus FA4-EGFP expressing EGFP-Fiber-2 fusion protein using the CRISPR/Cas9 technique, which showed significant attenuation compared to wild type FAdV-4 and provided protection against lethal challenge in chickens. Additionally, FA4-EGFP induced neutralizing antibodies with higher titers earlier than an inactivated vaccine, suggesting its potential as a live-attenuated vaccine candidate against highly pathogenic FAdV-4.

VETERINARY RESEARCH (2021)

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Article Immunology

Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4

Quan Xie et al.

Summary: This study identified a key interaction between Fiber-2 and KPNA3/4, demonstrating the role of KPNA3/4 in assisting FAdV-4 replication. A CRISPR-Cas9 edited virus with a deletion in Fiber-2 showed high attenuation and provided full protection against wild type FAdV-4, offering potential targets for antiviral strategies and vaccine development.

VIRULENCE (2021)

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Article Immunology