4.6 Article

Human Transcriptome Array Analysis Identifies CDR2 as a Novel Suppressed Gene for Kawasaki Disease

Journal

DIAGNOSTICS
Volume 12, Issue 2, Pages -

Publisher

MDPI
DOI: 10.3390/diagnostics12020240

Keywords

CDR2; Kawasaki disease; suppressed gene

Funding

  1. Ministry of Science and Technology of Taiwan [MOST 108-2314-B-182 -037 -MY3, 110-2314-B-182A-035 -MY3]
  2. Chang Gung Memorial Hospital [CMRPG8L0021, CMRPG8L0031, CMRPG8L0041]

Ask authors/readers for more resources

This study focused on suppressed genes in the acute stage of Kawasaki disease (KD) and identified novel targets with clinical significance and potential prognostic value. By analyzing human transcriptome arrays, the researchers identified CDR2 as a suppressed gene in KD and found that DDX24 is associated with CAL formation.
Kawasaki disease (KD) is a febrile childhood vasculitis that involves the coronary arteries. Most previous studies have focused on the genes activated in the acute phase of KD. However, in this study, we focused on suppressed genes in the acute stage of KD and identified novel targets with clinical significance and potential prognostic value for KD patients. We enrolled 18 patients with KD, 18 healthy controls (HC), and 18 febrile controls (FC) for human transcriptome array analysis. Another 19 healthy controls, 20 febrile controls, and 31 patients with KD were recruited for RT-PCR validation of target mRNA expressions. The results of Human Transcriptome Array (HTA) 2.0 showed 461 genes that were significantly higher in KD and then normalized after IVIG, as well as 99 suppressed genes in KD. Furthermore, we identified the four genes in KD with the most downregulation, including BCL11B, DUSP2, DDX24, and CDR2, as well as the upregulation of their expression following IVIG administration. The mRNA expression of CDR2 by qRT-PCR was the most compatible with the pattern of the HTA2.0 results. Furthermore, we found higher DDX24 mRNA expression in KD patients with CAL when compared to those without CAL 3 weeks after IVIG administration. In summary, activated gene expression represented a majority in the immune response of KD. In this study, we identified CDR2 as a novel suppressed gene for Kawasaki disease via human transcriptome array analysis and DDX24 associated with CAL formation, which may contribute to further understanding of CAL pathogenesis in KD.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available