Seed Dormancy Class and Ecophysiological Features of Veronicastrum sibiricum (L.) Pennell (Scrophulariaceae) Native to the Korea Peninsula

Veronicastrum sibiricum is a perennial species distributed in Korea, Japan, Manchuria, China, and Siberia. This study aimed to determine the requirements for germination and dormancy break of V. sibiricum seeds and to classify the kind of seed dormancy. Additionally, its class of dormancy was compared with other Veronicastrum and Veronica species. V. sibiricum seeds were permeable to water and had a mature embryo during seed dispersal. In field conditions, germination was prevented by physiological dormancy, which was, however, relieved by March of the next year, allowing the start of germination when suitable environmental conditions occurred. In laboratory experiments, the seeds treated with 0, 2, 4, 8, and 12 weeks of cold stratification (4 degrees C) germinated to 0, 79, 75, 72, and 66%, respectively. After the GA(3) treatment (2.887 mM), >= 90% of the seeds germinated during the four incubation weeks at 20/10 degrees C. Thus, 2.887 mM GA(3) and at least two weeks at 4 degrees C were effective in breaking physiological dormancy and initiating germination. Therefore, the V. sibiricum seeds showed non-deep physiological dormancy (PD). Previous research, which determined seed dormancy classes, revealed that Veronica taxa have PD, morphological (MD), or morphophysiological seed dormancy (MPD). The differences in the seed dormancy classes in the Veronicastrum-Veronica clade suggested that seed dormancy traits had diverged. The results provide important data for the evolutionary ecological studies of seed dormancy and seed-based mass propagation of V. sibiricum.

Automated summary

This study investigates the germination and dormancy break requirements of Veronicastrum sibiricum seeds and compares their dormancy class with other Veronicastrum and Veronica species. The results show that physiological dormancy prevents germination in the field, but is relieved in March of the next year. Laboratory experiments reveal that cold stratification and GA(3) treatment effectively break physiological dormancy and initiate germination. The study also highlights the divergent dormancy traits in the Veronicastrum-Veronica clade.