Morphological damage and increased ROS production of biosynthesized silver nanoparticle against MCF-7 breast cancer cells through in vitro approaches

In this study of anti-cancer activity, the cytotoxicity effect of the silver nanoparticle was shown excellent anti-cancer activity against MCF-7 breast cancer cells. The IC50 concentration of the synthesized silver nanoparticle against performed MCF-7 breast cancer cells was 1000 mg/ml concentration. Further, the shape modifications in the silver nanoparticle treated MCF-7 cells were viewed by phase contrast micro-scope and proved after comparison with untreated control. Next, the intracellular proliferation, chro-matin condensation, granular membranes cleavage and surface layers damage were effectively seen by fluorescence microscopy using AO/EB fluorescence dyes. The failure of matured nucleus was clearly shown after the treatment of Ag NPs and was effectively proved by fluorescence microscope using Hoechst 33342 stain. Finally, the anti-cancer effect identification through various invitro experiments was clearly favored to performed silver nanoparticle, and suggested that the nanoparticle was very effec-tive against multi drug resistant bacteria, biofilm producing bacteria and cancer cells. (c) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

In this study, the excellent anti-cancer activity of silver nanoparticles against MCF-7 breast cancer cells was demonstrated. The nanoparticles induced cytotoxicity, morphological changes, and damage to intracellular structures.