Journal
ANTIBIOTICS-BASEL
Volume 11, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics11010117
Keywords
dairy cow mastitis; cephalosporin; fecal microbiota; antibiotic resistance genes
Categories
Funding
- Scientific Research Project for Major Achievements of Agricultural Science and Technology Innovation Program (ASTIP) [CAAS-ZDXT2019004]
- Agricultural Science and Technology Innovation Program [ASTIP-IAS12]
- China Agriculture Research System of MOF
- Project of Risk Assessment on Raw Milk [GJFP2019027]
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The effects of ceftiofur and cefquinome on the fecal microbiota and antibiotic resistance genes of dairy cows with mastitis were investigated in this study. The results showed that cephalosporin treatment significantly decreased microbial diversity and richness, and increased the relative abundance of certain bacterial taxa and two beta-lactam resistance genes.
Antibiotics are frequently used to treat dairy cows with mastitis. However, the potential effects of beta-lactam antibiotics, such as cephalosporins, on the fecal microbiome is unknown. The objective was to investigate the effects of ceftiofur and cefquinome on the fecal microbiota and antibiotic resistance genes of dairy cows with mastitis. The fecal samples were collected from 8 dairy cows at the following periods: the start day (Day 0), medication (Days 1, 2, and 3), withdrawal (Days 4, 6, 7, and 8), and recovery (Days 9, 11, 13, and 15). 16S rRNA gene sequencing was applied to explore the changes in microbiota, and qPCR was used to investigate the antibiotic resistance genes. The cephalosporin treatment significantly decreased the microbial diversity and richness, indicated by the decreased Shannon and Chao 1 indexes, respectively (p < 0.05). The relative abundance of Bacteroides, Bacteroidaceae, Bacteroidales, and Bacteroidia increased, and the relative abundance of Clostridia, Clostridiales, Ethanoligenens, and Clostridium IV decreased at the withdrawal period. The cephalosporin treatment increased the relative abundance of beta-lactam resistance genes (bla(TEM) and cfxA) at the withdrawal period (p < 0.05). In conclusion, the cephalosporin treatment decreased the microbial diversity and richness at the medication period, and increased the relative abundance of two beta-lactam resistance genes at the withdrawal period.
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