Journal
ANTIBIOTICS-BASEL
Volume 10, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics10111374
Keywords
bla (CTX-M); carbapenemase; pet; clones; uropathogenic Escherichia coli
Categories
Funding
- Chulalongkorn University to the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksompoch Endowment Fund), Chulalongkorn Academic Advancement into its 2nd Century Project
- Center of Excellence for the Emerging and Re-emerging Infectious Diseases in Animals (CUEIDAs) and the One Health Research Cluster
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This study characterized the genetic features of ESC-resistant E. coli isolated from cats and dogs with urinary tract infections in Thailand, revealing multiple antimicrobial resistance genes and a high prevalence of multidrug-resistant phenotypes. Additionally, the presence of various high-risk ExPEC clones suggests potential clonal dissemination in these animal reservoirs.
Resistance to extended-spectrum cephalosporins (ESC) and carbapenems in Escherichia coli (E. coli), increasingly identified in small animals, indicates a crisis of an antimicrobial resistance situation in veterinary medicine and public health. This study aimed to characterise the genetic features of ESC-resistant E. coli isolated from cats and dogs with urinary tract infections in Thailand. Of 72 ESC-resistant E. coli isolated from diagnostic samples (2016-2018), bla(CTX-M) including group 1 (CTX-M-55, -15 and -173) and group 9 (CTX-M-14, -27, -65 and -90) variants were detected in 47 isolates (65.28%) using PCR and DNA sequencing. Additional antimicrobial resistance genes, including plasmid-mediated AmpC (CIT and DHA), bla(NDM-5), mcr-3, mph(A) and aac(6 & PRIME;)-Ib-cr, were detected in these isolates. Using a broth microdilution assay, all the strains exhibited multidrug-resistant phenotypes. The phylogroups were F (36.11%), A (20.83%), B1 (19.44%), B2 (19.44%) and D (4.17%), with several virulence genes, plasmid replicons and an integrase gene. The DNA fingerprinting using a repetitive extragenic palindromic sequence-PCR presented clonal relationships within phylogroups. Multiple human-associated, high-risk ExPEC clones associated with multidrug resistance, including sequence type (ST) 38, ST131, ST224, ST167, ST354, ST410, ST617 and ST648, were identified, suggesting clonal dissemination. Dogs and cats are a potential reservoir of ESC-resistant E. coli and significant antimicrobial resistance genes.
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