Journal
FOODS
Volume 11, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/foods11010041
Keywords
krill oil; Fourier-transform infrared spectroscopy; second-derivative spectrum; phospholipids; phosphatidylcholine
Categories
Funding
- Chung-Ang University Graduate Research Scholarship in 2020 [20162MFDS038]
- Ministry of Food and Drug Safety, Korea
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This study developed a FT-IR spectroscopy method for quantifying phosphatidylcholine and total phospholipid content in krill oil. The method showed good accuracy, repeatability, and can be used as a convenient and rapid alternative to traditional methods.
This study focuses on developing a quantification method for phosphatidylcholine (PC) and total phospholipid (PL) in krill oil using Fourier-transform infrared (FT-IR) spectroscopy. Signals derived from the choline and phosphate groups were selected as indicator variables for determining PC and total PL content; calibration curves with a correlation coefficient of >0.988 were constructed with calibration samples prepared by mixing krill oil raw material and fish oil in different ratios. The limit of detection (LOD, 0.35-3.29%) of the method was suitable for the designed assay with good accuracy (97.90-100.33%). The relative standard deviations for repeatability (0.90-2.31%) were acceptable. Therefore, both the methods using absorbance and that using second-derivative were confirmed to be suitable for quantitative analysis. When applying this method to test samples, including supplements, the PC content and total PL content were in good agreement with an average difference of 2-3% compared to the P-31 NMR method. These results confirmed that the FT-IR method can be used as a convenient and rapid alternative to the P-31 NMR method for quantifying PLs in krill oil.
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