Journal
FOODS
Volume 11, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/foods11030429
Keywords
phenolic compounds; glucosinolates; Response Surface Methodology; proteins; Brassica carinata
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Funding
- National Institute of Food and Agriculture, United States Department of Agriculture [2016-11231]
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The extraction of secondary metabolites from Brassica byproducts using aqueous ethanol extraction at low ethanol concentration and moderate temperature can efficiently recover the metabolites while maintaining good protein extraction. Under optimized conditions, aqueous extraction can also improve the functional properties of the protein isolates.
The sustainable extraction of secondary metabolites from Brassica agro-industrial byproducts often involves the use of high concentrations of ethanol, and/or high temperatures, which tends to decrease the efficiency of protein extraction (yield, profile, etc.). To understand the limits of the combination of these two extraction processes, aqueous ethanol extraction of secondary metabolites (e.g., phenolic compounds and glucosinolates) from Brassica carinata defatted meal was optimized using Response Surface Methodology. The validated models predicted that aqueous ethanol extraction of defatted Carinata meal, with a low aqueous EtOH concentration (22% EtOH) at moderate T-e (50 degrees C), enables the efficient recovery of secondary metabolites (sinapine = 9.12 +/- 0.05 mg/gDM, sinigrin = 86.54 +/- 3.18 mu mol/gDM) while maintaining good protein extractability (59.8 +/- 2.1%) from successive alkaline extractions. The evaluation of functional properties of the resulting protein isolates revealed that aqueous extraction, under optimized conditions, improves foaming activity while preserving emulsion ability.
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