4.7 Article

Optimization of Solid-State Fermentation Extraction of Inonotus hispidus Fruiting Body Melanin

Journal

FOODS
Volume 10, Issue 12, Pages -

Publisher

MDPI
DOI: 10.3390/foods10122893

Keywords

melanin; optimization; structure; Inonotus hispidus

Funding

  1. Research and Application of Key Techniques for High-yield and High-quality Cultivation of sanghuang [2019N0006, XZ202001ZY0041N]

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The study found that the compound enzymatic method was the best way to extract IHFM, with the best solid medium formula being normal pH, rice 20 g per cultivation bottle, maltose 22 g/L, beef extract 4.4 g/L, carbon-nitrogen ratio 5:1, and liquid-to-material ratio 1.1:1, resulting in significantly improved yield of melanin.
Melanin has good nutritional and medicinal value; however, its extraction rate is extremely low. This study explored the edible and medicinal fungus Inonotus hispidus fruiting body melanin (IHFM) extraction process and solid-state fermentation conditions. The results showed that the best way to extract IHFM is the compound enzymatic method, with complex enzyme 26.63 mg/g, liquid material ratio 5:1, enzymatic hydrolysis 80 min, pH 4.61, and enzymolysis temperature at 36.07 degrees C. The yield of IHFM was 23.73 +/- 0.57%, which was equivalent to 1.27 times before optimization. The best solid medium formula was normal pH, rice 20 g per cultivation bottle, maltose 22 g/L, beef extract 4.4 g/L, carbon-nitrogen ratio 5:1, and liquid-to-material ratio 1.1:1, where the IHFM yield was 31.80 +/- 1.34%, which was equivalent to 1.7 times that before optimization. In summary, solid-state fermentation and extraction optimization greatly improved the yield of melanin, provided a reference to produce melanin, and laid a foundation for the development and utilization of melanin.

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