Journal
FOODS
Volume 11, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/foods11040595
Keywords
DNA-stabilized silver nanoclusters; gold nanoparticles; magnetic beads; fluorescence aptasensor; Salmonella Typhimurium
Categories
Funding
- National Natural Science Foundation of China [U21A20272]
- Natural Science Foundation of Heilongjiang Province of China [ZD2021C004]
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In this study, a novel fluorescence aptasensor was developed for rapid and sensitive detection of Salmonella Typhimurium (S. Typhimurium). The aptasensor exhibited high selectivity and sensitivity, and showed excellent application for detection in artificially contaminated milk.
Salmonella Typhimurium (S. Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of S. Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect S. Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a sandwich structure was established. The aptamer acted as a link, and its specific binding to S. Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect S. Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to S. Typhimurium, ranging from 3.7 x 10(2) to 3.7 x 10(5) cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of S. Typhimurium in artificially contaminated milk, with LOD reaching 3.4 x 10(2) cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify S. Typhimurium in foodstuffs.
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