Journal
FOODS
Volume 10, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/foods10112647
Keywords
aristolochic acid I; monoclonal antibody; computer-assisted simulation; chemiluminescent immunoassay; fluoroimmunoassay
Categories
Funding
- Guangzhou Science and Technology Foundation [201903010034]
- Natural Resources Science Foundation of Guangdong Province [2018A030313926]
- Science and Technology Foundation Key R&D Program of Guangdong Province [2019B020209009, 2019B020218009]
- R&D Program of Guangdong Province Drug Administration [2021TDZ09, 2021YDZ06]
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This study developed a CLEIA for AA-I using a high-sensitivity mAb and improved the detection sensitivity of FIA by using rCDs in icELISA. The sensitivity of FIA was improved by five-fold compared to CLEIA, and the proposed assays showed good accuracy and practicability.
Aristolochic acid (AA) toxicity has been shown in humans regarding carcinogenesis, nephrotoxicity, and mutagenicity. Monitoring the AA content in drug homologous and healthy foods is necessary for the health of humans. In this study, a monoclonal antibody (mAb) with high sensitivity for aristolochic acid I (AA-I) was prepared. Based on the obtained mAb, a chemiluminescent immunoassay (CLEIA) against AA-I was developed, which showed the 50% decrease in the RLUmax (IC50) value of 1.8 ng/mL and the limit of detection (LOD) of 0.4 ng/mL. Carbon dots with red emission at 620 nm, namely rCDs, were synthesized and employed in conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) to improve the assay sensitivity of a fluoroimmunoassay (FIA). Oxidized 3,3 & PRIME;& PRIME;,5,5 & PRIME;& PRIME;-tetramethylbenzidine dihydrochloride (oxTMB) can quench the emission of the rCDs through the inner-filter effect; therefore, the fluorescence intensity of rCDs can be regulated by the concentration of mAb. As a result, the assay sensitivity of FIA was improved by five-fold compared to CLEIA. A good relationship between the results of the proposed assays and the standard ultra-high performance liquid chromatography-triple quadrupole mass spectrometer (UPLC-QQQ-MS/MS) of real samples indicated good accuracy and practicability of CLEIA and FIA.
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