4.7 Article

Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites

Journal

FOODS
Volume 11, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/foods11040526

Keywords

hydroxytyrosol; VEGF; eNOS; metabolites; 3; 4-dihydroxyphenylacetaldehyde; indole pyruvic acid; angiogenesis

Funding

  1. Junta de Andalucia [P18-RT-3098]
  2. Ministry of Science, In-novation and Universities
  3. [PID2019-108722RB-C32]
  4. [MCIN/AEI/10.13039/501 100011033]

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This study revealed the molecular mechanism underlying the inhibition of VEGF signaling by hydroxytyrosol metabolites and indolic compounds, and established a relationship between their structure and bioactivity. The compounds exhibited anti-angiogenic effects by regulating signaling pathways such as PLC gamma 1 and eNOS.
Angiogenesis is a key process involved in both cancer and cardiovascular diseases, the vascular endothelial growth factor (VEGF) and its VEGF receptor-2 (VEGFR-2) being the main triggers. The aim of this study was to determine the molecular mechanism underlying the potent inhibition of VEGF signaling by hydroxytyrosol (HT) metabolites and indolic compounds and establish a relation between their structure and bioactivity. Experiments involved the evaluation of their potential to inhibit VEGF on human umbilical vein endothelial cells (HUVECs) by ELISA assay and their subsequent effect on the downstream signaling pathway (PLC gamma 1, Akt, and endothelial nitric oxide synthetase (eNOS)) by Western blot. Respectively, 3,4-dihydroxyphenylacetaldehyde (DOPAL) (100 mu M) and indole pyruvic acid (IPy) (1 mM) were capable of inhibiting VEGFR-2 activation with an IC50 value of 119 mu M and 1.037 mM. The anti-angiogenic effect of DOPAL and IPy is mediated via PLC gamma 1. Additionally, DOPAL significantly increases eNOS phosphorylation, while IPy maintained it. These data provide for the first time evidence of the anti-angiogenic effect of DOPAL and IPy for future use as potential bioactive food ingredients.

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