Journal
FOODS
Volume 11, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/foods11030420
Keywords
Moringa oleifera; oxidative stress; antioxidants; Vero; polyphenols
Categories
Funding
- Small and Medium Business Administration [S2838176]
Ask authors/readers for more resources
The present study identified phenolic compounds in Moringa oleifera hot water extract (MOH) and evaluated its antioxidant activity on H2O2-induced oxidative stress in Vero cells. Results showed that MOH exhibited strong radical scavenging and antioxidant properties, reduced ROS generation, increased cell viability, suppressed mitochondrial depolarization and apoptosis, and activated the Nrf2/HO-1 signaling pathway.
The present study discloses the identification of phenolic compounds in Moringa oleifera hot water extract (MOH) and the evaluation of its antioxidant activity on H2O2-induced oxidative stress in Vero cells. Upon analysis, MOH was found to contain phenolic compounds and indicated 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(+)) radical scavenging with IC50 values of 102.52 and 122.55 mu g/mL, respectively. The ferric reducing antioxidant power (FRAP) of MOH indicated a dose-dependent increase with a maximum absorbance at 125 mu g/mL and the oxygen radical absorbance capacity (ORAC) of MOH was 1004.95 mu mol TE/mg. Results showed that MOH dose-dependently reduced intracellular ROS generation in H2O2-stimulated Vero cells while increasing the cell viability. Fluorescence microscopy and flowcytometric analyses have supported the above findings. MOH markedly suppressed the H2O2-induced mitochondrial depolarization and apoptosis through suppression of the mitochondrial-mediated apoptosis pathway and activated the Nrf2/HO-1 signaling pathway by possibly involving H2O2 generation in cell media. Findings of western blot were supported by immunocytochemistry of Nrf2 nuclear translocation. Thus, MOH bioactivity would potentiate its applications in manufacturing functional food.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available