Journal
NATURE AND SCIENCE OF SLEEP
Volume 14, Issue -, Pages 121-133Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/NSS.S334985
Keywords
gut microbiota; sleep deprivation; short-chain fatty acid; function prediction
Categories
Funding
- National Key Research and Development Program of China [2020YFC2003600]
- National Natural Science Foundation of China [81901347, 82171477, 31800897, 81821092, 82001404]
- Peking University Medicine Fund for Fostering Young Scholars' Scientific and Technological Innovation
- Fundamental Research Funds for Central Universities [BMU2018PYB010]
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The study reveals that complete sleep deprivation leads to significant changes in gut microbial composition and function, but these changes can be reversed.
Introduction: Accumulating evidence suggests that both sleep loss and gut dysbiosis can lead to metabolic disorders. However, less is known about the impact of total sleep deprivation (SD) and sleep recovery on the composition, function, and metabolic dynamics of the gut microbiota. Methods: Specific-pathogen free Sprague-Dawley rats were subjected to 48 h of SD with gentle handling and then allowed to recover for 1 week. Taxonomic profiles of fecal microbiota were obtained at baseline, 24 h of SD, 48 h of SD, and 1 week of recovery. We used 16S rRNA gene sequencing to analyze the gut microbial composition and function and further characterize microbiota-derived metabolites in rats. Results: The microbiota composition analysis revealed that gut microbial composition and metabolites did not change in the rats after 24 h of SD but were significantly altered after 48 h of SD. These changes were reversible after 1 week of sleep recovery. A functional analysis was performed based on Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations, indicating that 19 KEGG pathways were significantly altered in the gut microbiota in SD rats. These functional changes occurred within 24 h of SD, were more apparent after 48 h of SD, and did not fully recover after 1 week of sleep recovery. Conclusion: These results indicate that acute total SD leads to significant compositional and functional changes in the gut microbiota, and these changes are reversible.
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