Journal
METABOLITES
Volume 12, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/metabo12020188
Keywords
Amaryllidaceae alkaloids; Phycella; cholinesterase; tyrosinase; antiproliferative; UHPLC-MS
Categories
Ask authors/readers for more resources
This research identifies the alkaloid profile of the Amaryllidaceae plant Phycella cyrtanthoides and evaluates its enzyme inhibition and antiproliferative effects. The study reveals that P. cyrtanthoides contains diverse bioactive alkaloids with potential therapeutic applications for neurodegenerative diseases.
This research aims to identify the alkaloid profile and to evaluate the enzyme inhibitory potential and antiproliferative effects of the Amaryllidaceae plant Phycella cyrtanthoides. The alkaloid extracts from bulbs and leaves were analyzed using ultrahigh performance liquid chromatography orbitrap mass spectrometry (UHPLC-Orbitrap-MS) analysis. A total of 70 alkaloids were detected in the P. cyrtanthoides' extracts. The enzyme inhibition potential against cholinesterases (AChE: acetylcholinesterase, and BChE butyrylcholinesterase) and tyrosinase were studied. Bulbs displayed the best IC50 values against AChE (4.29 +/- 0.03 mu g/mL) and BChE (18.32 +/- 0.03 mu g/mL). These results were consistent with docking experiments with selected major compounds in the active sites of enzymes, while no activity was observed against tyrosinase enzyme. Antiproliferative effects were investigated against human cervical (HeLa), lung (A549, SW1573), colon (WiDr), and breast (HBL-100, T-47D) tumor cell lines. Bulbs and leaves were active in all cell lines (GI(50) < 2.5 mu g/mL). These findings suggest that the endemic Chilean plant P. cyrtanthoides contains diverse types of bioactive alkaloids with antiproliferative activities and inhibitory effects with potential therapeutic applications for neurodegenerative diseases
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available