Journal
METABOLITES
Volume 12, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/metabo12020148
Keywords
H-1-NMR; metabolomics; feces; intestine; enteric; microbiome; rats; chickens
Categories
Funding
- Canadian Institutes of Health Research (CIHR) [363195]
- Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant [5519]
- Alberta Agriculture and Forestry [2019F101R]
- Canadian Poultry Research Council (CPRC) Poultry Science Cluster Project [1373 Activity 14]
Ask authors/readers for more resources
Analysis of fecal metabolome can provide valuable diagnostic information about enteric microbiota function. However, the presence of macromolecules in fecal samples hinders accurate analysis. This study tested three methods and found that ultrafiltration effectively removes macromolecules, enabling accurate analysis.
Analysis of enteric microbiota function indirectly through the fecal metabolome has the potential to be an informative diagnostic tool. However, metabolomic analysis of feces is hampered by high concentrations of macromolecules such as proteins, fats, and fiber in samples. Three methods-ultrafiltration (UF), Bligh-Dyer (BD), and no extraction (samples added directly to buffer, vortexed, and centrifuged)-were tested on multiple rat (n = 10) and chicken (n = 8) fecal samples to ascertain whether the methods worked equally well across species and individuals. An in silico baseline correction method was evaluated to determine if an algorithm could produce spectra similar to those obtained via UF. For both rat and chicken feces, UF removed all macromolecules and produced no baseline distortion among samples. By contrast, the BD and no extraction methods did not remove all the macromolecules and produced baseline distortions. The application of in silico baseline correction produced spectra comparable to UF spectra. In the case of no extraction, more intense peaks were produced. This suggests that baseline correction may be a cost-effective method for metabolomic analyses of fecal samples and an alternative to UF. UF was the most versatile and efficient extraction method; however, BD and no extraction followed by baseline correction can produce comparable results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available