Journal
PATHOGENS
Volume 11, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/pathogens11020229
Keywords
oral squamous cell carcinoma; saliva; miR-345; miR-31; miR-424; real-time qPCR
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Funding
- Hungarian Scientific Research Fund (OTKA) [105034]
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Early detection of oral squamous cell carcinoma (OSCC) is crucial for prognosis. Saliva, as a comprehensive sample, shows promising potential for OSCC detection. The study identified miR-345 and miR-31-5p as consistently upregulated salivary biomarkers for OSCC and a three-miRNA panel showed high sensitivity in distinguishing cancer and control patients.
If not detected early, oral squamous cell carcinoma (OSCC) has very poor prognosis, emphasizing the need for reliable early diagnostics. Saliva is considered a promising surrogate biosample for OSCC detection, because it comes into contact with many cells of the tumor mass, providing a comprehensive sampling of tumor-specific biomolecules. Although several protein- and RNA-based salivary biomarkers have been proposed for the detection of OSCC, the results of the studies show large differences. Our goal was to clarify which salivary microRNAs (miRNA) show reliably high expression in the saliva of OSCC patients, to be used as cancer-specific biomarkers, and potentially as early diagnostic biomarkers. Based on a detailed literature search, we selected six miRNAs commonly overexpressed in OSCC, and analyzed their expression in saliva samples of cancer patients and controls by real-time quantitative PCR. Our results suggest that miR-345 and miR-31-5p are consistently upregulated salivary biomarkers for OSCC, and a three-miRNA panel of miR-345, miR-31-5p, and miR-424-3p can distinguish cancer and control patients with high sensitivity.
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