Journal
INTERNATIONAL JOURNAL OF GREEN ENERGY
Volume 13, Issue 3, Pages 292-297Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15435075.2014.893436
Keywords
Biological hydrogen production; fermentation; PCR-DGGE; pre-treatment; seawater
Funding
- Key Natural Science Foundation of Tianjin of China [14JCZDJC40200]
- Science Foundation of Tianjin University of Science Technology [20130114]
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Batch tests of cultivations were conducted to analyze influences of pre-treatment methods (heat-shock, acid, and alkaline) on hydrogen production. The hydrogen yields of the pre-treated samples were significantly higher than those without pre-treatment. Heat-shock pre-treatment yielded maximum hydrogen (75.5 +/- 4.0 mL/g sucrose(removed)), and had the highest sucrose removal efficiency (81.20 +/- 1.23%). Heat-shock pre-treatment produced more ethanol and acetate (80.44 +/- 0.55% of the total soluble metabolites), which belonged to ethanol-type fermentation. Denaturing gradient gel electrophoresis (DGGE) profiles showed that pre-treatment methods affected the composition of microbial communities. The results showed that pre-treatment methods play a key role in the hydrogen production process of marine hydrogen-producing microflora.
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