Journal
MICROORGANISMS
Volume 10, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms10010135
Keywords
voles; wild rodents; tuberculosis; Mycobacterium microti; experimental infection
Categories
Ask authors/readers for more resources
A laboratory infection model was established to reproduce Mycobacterium microti infection in bank voles, successfully mimicking the disease phenotype observed in free-ranging voles. The pathogenesis of the infection was characterized, with most voles showing multifocal and diffuse granulomatous lesions in the liver and spleen. Mycobacterial DNA was detected in feces but not in oral swabs, suggesting horizontal transmission between voles.
Voles are maintenance hosts of Mycobacterium microti. In line with the goal to eradicate tuberculosis (TB) in livestock, the role of this mycobacteria needs to be assessed since it might interfere with current M. bovis/M. caprae surveillance strategies. To better understand the pathogenesis of TB in voles, an experimental infection model was set up to reproduce M. microti infection in laboratory Bank voles (Myodes glareolus). Two infection routes (intragastric and intraperitoneal) and doses (10(5) and 10(6) CFU/0.1 mL) were assessed. Voles were culled at different post-infection time points. Serology, histopathology, acid-fast bacilli staining, qPCR, and mycobacterial culture from tissues were performed. In addition, qPCR from feces and oral swabs were conducted to assess bacterial shedding. The model allowed us to faithfully reproduce the disease phenotype described in free-ranging voles and characterize the pathogenesis of the infection. Most animals showed multifocal and diffuse granulomatous lesions in the liver and spleen, respectively. Less frequently, granulomas were observed in lungs, lymph nodes, muscles, and salivary gland. Mycobacterial DNA was detected in feces from a few animals but not in oral swabs. However, one contact uninfected vole seroconverted and showed incipient TB compatible lesions, suggesting horizontal transmission between voles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available