Journal
MICROORGANISMS
Volume 10, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms10010145
Keywords
antibacterial bioassay; Chlorella vulgaris; nanogold; nanosilver; RNA extraction
Categories
Funding
- Deanship of Scientific Research at King Faisal University [NA000170]
Ask authors/readers for more resources
This study investigated the production capacity and antimicrobial activity of silver and gold nanoparticles by Chlorella vulgaris from Al-Ahsa, KSA. The results showed that the silver nanoparticles had the strongest antibacterial effect. By isolating and observing the RNA changes, it was suggested that the synthesis of nanoparticles may involve both biotic and abiotic processes. The use of nanosilver particles as antibacterial agents is recommended.
Chlorella vulgaris from Al-Ahsa, KSA was proved to be an active silver and gold nanoparticle producer. Nanogold and nanosilver particles were characterized using UV-visible spectroscopy, Fourier-transform infrared spectroscopy, and scanning electronmicroscopy. Both nanoparticles were used in the antimicrobial bioassay. The two nanoparticles showed antibacterial activities, with the silver nanoparticles being the most effective. To investigate the argumentative nature of their biosynthesis (i.e., whether it is a biotic or abiotic process), we isolated total ribonucleic acid RNA as an indicator of vitality. RNA was completely absent in samples taken after one week of incubation with silver nitrate and even after one or two days. However, successful extraction was only achievable in samples taken after incubation for one and four hours with silver nitrate. Most importantly, the gel image showed recognizable shearing of the nucleic acid after 4 h as compared to the control. An assumption can be drawn that the synthesis of nanoparticles may start biotically by the action of enzyme(s) and abiotically by action of reducing entities. Nonetheless, with prolonged incubation, excessive nanoparticle accumulation can be deadly. Hence, their synthesis continues abiotically. From the RNA banding profile, we suggest that nanosilver production starts both biotically and abiotically in the first few hours of incubation and then continues abiotically. Nanosilver particles proved to have more of an antimicrobial impact than nanogold and hence are recommended for different applications as antibacterial agents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available