Journal
MICROORGANISMS
Volume 10, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms10020471
Keywords
lactobacilli strains mixture; Gardnerella vaginalis; bacterial vaginosis; epithelial exfoliation
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Funding
- Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the Regional Star-Enterprise Development Program - Korea Institute for Advancement of Technology (KIAT) [P0010059]
- Korea Technology & Information Promotion Agency for SMEs (TIPA) [P0010059] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study investigated the effects of a mixture of five lactobacilli strains on bacterial vaginitis (BV) in HeLa cells and GV-infected BV mice. The findings suggest that this mixture can inhibit the growth of GV, reduce inflammation, and suppress the exfoliation of vaginal epithelial cells.
The present study investigated the anti-bacterial vaginitis (BV) effects of a mixture of five lactobacilli strains (LM5), containing equal amounts of Ligilactobacillus salivarius MG242, Limosilactobacillus fermentum MG901, Lactiplantibacillus plantarum MG989, Lacticaseibacillus paracasei MG4272, and Lacticaseibacillus rhamnosus MG4288), in HeLa cells and Gardnerella vaginalis (GV)-infected BV mice. All strains produced lactic acid and hydrogen peroxide, and were resistant to nonoxynol-9. LM5 significantly inhibited GV growth by 80%, exhibited good adhesion to HeLa cells, and significantly inhibited GV adhesion to these cells. In GV-infected mice, LM5 administered orally at 5 x 10(9) CFU/mouse significantly inhibited GV proliferation in the vaginal tract and significantly reduced myeloperoxidase activity, pro-inflammatory cytokine (TNF-alpha, IL-1 beta, and IL-6) levels, and nitric oxide levels in vaginal tissue lysates. Histopathological analysis of vaginal tissues revealed that LM5 markedly suppressed the exfoliation of vaginal epithelial cells. Overall, these results suggest that LM5 might alleviate BV by direct antibacterial and antagonistic activity in vaginal tissues of GV-infected mice.
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