Journal
MICROORGANISMS
Volume 9, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9112285
Keywords
autochthonous bioremediation; oil spills; hydrocarbons; bioaugmentation; bioremediation agent; lyophilized bacteria; biotechnology; marine environment
Categories
Funding
- project BIOREM-Bioremediation of hydrocarbon pollutants by autochthonous microorganisms in aquatic environment [PTDC/BTA-GES/32186/2017, POCI-01-0145-FEDER-032186]
- Fundo Europeu de Desenvolvimento Regional (FEDER) through COMPETE2020-Programa Operacional da Competitividade e Internacionalizacao (POCI)
- FCT/MCTES by national funds through FCT-Foundation for Science and Technology [UIDB/04423/2020, UIDP/04423/2020]
- FCT [2020.04689.BD]
- Fundação para a Ciência e a Tecnologia [2020.04689.BD, PTDC/BTA-GES/32186/2017] Funding Source: FCT
Ask authors/readers for more resources
This study developed a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Results showed that the lyophilized bacterial consortium significantly accelerated oil degradation and removal, indicating its potential for application in autochthonous oil bioremediation.
This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of Rhodococcus erythropolis and one Pseudomonas sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available