4.6 Article

Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit

Journal

MICROORGANISMS
Volume 10, Issue 2, Pages -

Publisher

MDPI
DOI: 10.3390/microorganisms10020425

Keywords

biocontrol; Pseudomonas syringae pv; actinidiae; Botrytis cinerea; DAPG; cyclic lipopeptides

Categories

Funding

  1. Key Research and Development Program of Zhejiang Province, China [2020C02006]
  2. Zhejiang Provincial Natural Science Foundation of China [LY19C010007]
  3. Shanghai Agricultural Basic Research Project [2019-02-08-00-08-F01150]
  4. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, China [2010DS700124-ZZ2014, 2010DS700124-KF202101]
  5. Taif University, Saudi Arabia [TURSP-2020/142]
  6. Taif University Researchers Supporting Project [TURSP-2020/142]
  7. [KF202101]

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An endophytic bacterium XL17 isolated from a rape crown gall showed potent antagonistic activities against Pseudomonas syringae pv. actinidiae (Psa) and Botrytis cinerea, the devastating diseases of kiwifruit worldwide. Strain XL17 belongs to Pseudomonas bijieensis and produces phenolic polyketide 2,4-diacetylphloroglucinol (DAPG) and cyclic lipopeptides, which can be used to control bacterial canker and gray mold pathogens of kiwifruit.
Kiwifruit worldwide suffers from the devastating diseases of bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) and gray mold caused by Botrytis cinerea. Here, an endophytic bacterium XL17 isolated from a rape crown gall was screened out for its potent antagonistic activities against Psa and B. cinerea. Strain XL17 and its cell-free culture filtrate (CF) inhibited the growth of Psa and B. cinerea, Psa-associated leaf necrosis, and B. cinerea-associated kiwifruit necrosis. Electron microscopy showed that XL17 CF could damage the cell structures of Psa and B. cinerea. Genome-based taxonomy revealed that strain XL17 belongs to Pseudomonas bijieensis within the P. corrugata subgroup of the P. fluorescens species complex. Among the P. corrugata subgroup containing 31 genomospecies, the presence of the phl operon responsible for the biosynthesis of the phenolic polyketide 2,4-diacetylphloroglucinol (DAPG) and the absence of the lipopeptide/quorum sensing island can serve as the genetic marker for the determination of a plant-protection life style. HPLC detected DAPG in extracts from XL17 CF. MALDI-TOF-MS analysis revealed that strain XL17 produced cyclic lipopeptides of the viscosin family and orfamide family. Together, phenotypic, genomic, and metabolic analyses identified that P. bijieensis XL17 producing DAPG and cyclic lipopeptides can be used to control bacterial canker and gray mold pathogens of kiwifruit.

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