4.7 Article

Anti-Inflammatory Potential of Complex Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout in Chronic Gingivitis: In Vitro Investigation and Randomized Clinical Trial

Journal

ANTIOXIDANTS
Volume 10, Issue 10, Pages -

Publisher

MDPI
DOI: 10.3390/antiox10101586

Keywords

gingivitis; medicinal plants; Ligularia stenocephala; Secale cereale

Funding

  1. Ministry of Agriculture, Food and Rural Affairs (MAFRA) [316054-3]
  2. Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET)

Ask authors/readers for more resources

The study demonstrated that TEES-10(R) significantly increased PDL cell viability in vitro and suppressed the expression of inflammatory mediators. In the clinical trial, oral administration of TEES-10(R) showed significant improvement in GI and BOP at 4 weeks, reducing the number of patients diagnosed with gingivitis.
Complex extracts of Ligularia stenocephala Matsum. & Koidz. (LSE) and Secale cereale L. sprout (SCSE) (TEES-10(R)) were prepared. The purposes of the study were to evaluate anti-inflammatory activities of TEES-10(R) in vitro and to observe resolution of gingivitis in human with oral administration of TEES-10(R). The effects of TEES-10(R) on normal periodontal ligament (PDL) cell viability, lipopolysaccharide (LPS) induced PDL cell viability and the changes of inflammatory mediator expression were evaluated in vitro. In the clinical trial, 150 mg of TEES-10(R) powder containing capsule was administered twice daily to the test group, while the control group administered placebos in a total 100 participants with gingivitis. Probing depth (PD), bleeding on probing (BOP), clinical attachment loss, gingival index (GI) and plaque index (PI) were measured at baseline and 4 weeks. Administering TEES-10(R) showed significant increase in PDL cell viability compared to administering LSE or SCSE alone. In addition, treating TEES-10(R) to LPS induced PDL cell significantly increased PDL cell viability compared to control. TEES-10(R) suppressed expression of NF-kappa B, p-ERK, ERK, COX-2, c-Fos and p-STAT and promoted expression of PPAR gamma in LPS induced PDL cells. In the clinical trial, significant improvement of GI and BOP was observed in the test group at 4 weeks. In addition, the number of patients diagnosed with gingivitis was significantly reduced in the test group at 4 weeks. Salivary MMP-8 and MMP-9 was also significantly decreased compared to placebo group. Within the limitations of this study, the TEES-10(R) would have an anti-inflammatory potential clinically in the chronic gingivitis patients.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available