Journal
ANTIOXIDANTS
Volume 11, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/antiox11020196
Keywords
oxidative stress; resveratrol; diastereomeric mixture; keratinocyte
Funding
- Region Emilia-Romagna (POR-FESR 2020)
- University of Ferrara [FIR 2020]
- 2021, Ferrara, Italy
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This study established a cellular model using fluorescent-engineered keratinocyte cell lines to investigate the protective effect of resveratrol against oxidant-induced cytotoxicity. The results demonstrated that cells pre-incubated with resveratrol were able to reverse the oxidative damage caused by the free radical promoter.
Resveratrol is a powerful antioxidant molecule. In the human diet, its most important source is in Vitis vinifera grape peel and leaves. Resveratrol exists in two isoforms, cis- and trans. The diastereomeric forms of many drugs have been reported as affecting their activity. The aim of this study was to set up a cellular model to investigate how far resveratrol could counteract cytotoxicity in an oxidant agent. For this purpose, a keratinocyte cell line, which was genetically engineered with jelly fish green fluorescent protein, was treated with the free radical promoter Cumene hydroperoxide. The antioxidant activity of the trans-resveratrol and its diastereomeric mixture was evaluated indirectly in these treated fluorescent-engineered keratinocytes by analyzing the cell number and cell proliferation index. Our results demonstrate that cells, which were pre-incubated with resveratrol, reverted the oxidative damage progression induced by this free radical agent. In conclusion, fluorescent-engineered human keratinocytes represent a rapid and low-cost cellular model to determine cell numbers by studying emitted fluorescence. Comparative studies carried out with fluorescent keratinocytes indicate that trans-resveratrol is more efficient than diastereomeric mixtures in protecting cells from the oxidative stress.
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