Journal
BIOMOLECULES
Volume 11, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/biom11101549
Keywords
hydrogen sulphide; ischemia-reperfusion injury; L-nitro-arginine-methyl-ester; ICAM-1; NF-kB; PAG
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Funding
- Institute of Postgraduate Studies (IPS), Universiti Sains Malaysia [P-FM0008/14(R)]
- Research and Creativity and Management Office (RCMO), Universiti Sains Malaysia
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Chronic administration of hydrogen sulphide donor (sodium hydrosulphide) improved renal ischemia-reperfusion injury in hypertensive rats, reducing blood pressure and oxidative stress markers while decreasing levels of ICAM-1 and NF-kB. The treatment with NaHS showed improvement in renal functional parameters and tubular morphology, indicating a potential antioxidant and anti-inflammatory mechanism.
Our main objective was to investigate the effect of chronic administration of hydrogen sulphide donor (sodium hydrosulphide) on the expression of intercellular adhesion molecule-1 (ICAM-1) and concentration of nuclear factor-kappa B (NF-kB) in a renal ischemia-reperfusion injury (IRI) model of WKY and L-nitro-arginine-methyl-ester (L-NAME)-induced hypertensive rats. Sodium hydrosulphide (NaHS) was administered intraperitoneally (i.p.) for 35 days while cystathionine gamma lyase (CSE) inhibitor dL-propargylglycine (PAG) was administered at a single dose of 50 mg/kg. Animals were anesthetised using sodium pentobarbitone (60 mg/kg) and then prepared to induce renal ischemia by clamping the left renal artery for 30 min followed by 3 h of reperfusion. Pre-treatment with NaHS improved the renal functional parameters in both WKY and L-NAME-induced hypertensive rats along with reduction of blood pressure in hypertensive groups. Oxidative stress markers like malondialdehyde (MDA), total superoxide dismutase (T-SOD) and glutathione (GSH) were also improved by NaHS treatment following renal IRI. Levels of ICAM-1 and NF-kB concentration were reduced by chronic treatment with NaHS and increased by PAG administration after renal IRI in plasma and kidney. Treatment with NaHS improved tubular morphology and glomerulus hypertrophy. Pre-treatment with NaHS reduced the degree of renal IRI by potentiating its antioxidant and anti-inflammatory mechanism, as evidenced by decreased NF-kB concentration and downregulation of ICAM-1 expression.
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