☆ 4.7 Article

The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous Expression

BIOMOLECULES (2021)

Related references

Note: Only part of the references are listed.

Article Biotechnology & Applied Microbiology

COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models

Antonella Conforti et al.

Summary: This study reports the preclinical evaluation of a novel COVID-19 vaccine candidate based on the electroporation of engineered, synthetic cDNA encoding a viral antigen in the skeletal muscle. The vaccine showed strong immunogenicity and significant protection in animal models, suggesting its potential as an ideal COVID-19 vaccine candidate for clinical development.

MOLECULAR THERAPY (2022)

Add to Collection

Article Biochemical Research Methods

Systemic effects of missense mutations on SARS-CoV-2 spike glycoprotein stability and receptor-binding affinity

Shaolei Teng et al.

Summary: This study quantified the systemic effects of missense mutations on the SARS-CoV-2 spike protein, revealing that most mutations destabilize the protein while specific mutations can enhance stability. The research also identified crucial residues affecting the RBD-ACE2 interaction, providing potential target sites for drug and vaccine development against COVID-19.

BRIEFINGS IN BIOINFORMATICS (2021)

Add to Collection

Article Genetics & Heredity

Application of newly developed SARS-CoV2 serology test along with real-time PCR for early detection in health care workers and on-time plasma donation

Mohammad Sadegh Soltani-Zangbar et al.

Summary: This study aimed to develop a serological test for early detection of anti-SARS-CoV-2 antibodies. Real-time PCR results showed some participants were infected with the virus, while the designed ELISA kit demonstrated high sensitivity and specificity, aiding in diagnosis of infection status and early screening.

GENE REPORTS (2021)

Add to Collection

Article Biophysics

Biomechanical characterization of SARS-CoV-2 spike RBD and human ACE2 protein-protein interaction

Wenpeng Cao et al.

Summary: This study utilizes single-molecule force spectroscopy and molecular dynamics simulations to quantify the interactions between SARS-CoV-2 or SARS-CoV-1 RBD and ACE2, revealing that the N-linked glycan interaction plays a crucial role in the binding difference between the two viruses. The study uncovers the mechanism behind ACE2 binding disparity and provides insights for developing new strategies to inhibit SARS-CoV-2 entry.

BIOPHYSICAL JOURNAL (2021)

Add to Collection

Editorial Material Medicine, General & Internal

SARS-CoV-2 Vaccines

C. Buddy Creech et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Nanoluciferase complementation-based bioreporter reveals the importance of N-linked glycosylation of SARS-CoV-2 S for viral entry

Taha Azad et al.

Summary: This study utilized a novel bioreporter technology to investigate the interaction between SARS-CoV-2 virus and its host cell entry receptor, identifying key host and viral factors, including the role of glycosylation of asparagine residues within the RBD in mediating successful viral entry.

MOLECULAR THERAPY (2021)

Add to Collection

Article Virology

Structure-Function Analyses of New SARS-CoV-2 Variants B.1.1.7, B.1.351 and B.1.1.28.1: Clinical, Diagnostic, Therapeutic and Public Health Implications

Jasdeep B. Singh et al.

Summary: The SARS-CoV-2 virus has undergone multiple mutations during its global circulation, leading to the emergence of three new lineages in the UK, South Africa, and Brazil. These mutations impact crucial proteins such as spike, ORF8, and nucleocapsid, potentially affecting host receptor affinity and antibody resistance.

VIRUSES-BASEL (2021)

Add to Collection

Review Biochemistry & Molecular Biology

Potential drug targets of SARS-CoV-2: From genomics to therapeutics

Anas Shamsi et al.

Summary: The emergence of SARS-CoV-2 has posed a global threat due to the continuous rise in COVID-19 cases. The complex mechanism of the virus pathogenesis hinders the development of effective therapeutics, but in-depth analysis of the genome architecture and pathogenesis may lead to potential treatment options.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Add to Collection

Article Biochemistry & Molecular Biology

Receptor binding, immune escape, and protein stability direct the natural selection of SARS-CoV-2 variants

Vaibhav Upadhyay et al.

Summary: The emergence of new SARS-CoV-2 variants has raised concerns about vaccine and antibody therapy effectiveness. This study examined the impact of 12 common mutations on spike protein in terms of expression, stability, activity, and antibody escape potential, revealing differences in affinity to receptors and antibodies among the mutants. Multiple factors contribute to natural selection of variants, emphasizing the need to consider various aspects to understand virus evolution.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Add to Collection

Article Biochemical Research Methods

Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies

Etienne Frumence et al.

Summary: The study developed an ELISA assay based on SARSCoV-2 antigens for detecting antibody levels in COVID-19 patients' plasma. The research demonstrated high reactivity against recombinant antigens was readily detected in all positive samples.

JOURNAL OF IMMUNOLOGICAL METHODS (2021)

Add to Collection

Article Plant Sciences

N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants

Yun-Ji Shin et al.

Summary: Studies on expressing glycosylated receptor binding domain (RBD) of SARS-CoV-2 spike protein in Nicotiana benthamiana revealed that a truncated RBD-215 variant can be efficiently expressed with glycosylation playing an important role in its functionality.

FRONTIERS IN PLANT SCIENCE (2021)

Add to Collection

Article Biochemistry & Molecular Biology

An overview of current COVID-19 vaccine platforms

Abdou Nagy et al.

Summary: The article discusses the development of SARS-CoV-2 vaccines, highlighting the numerous vaccine candidates in pre-clinical and clinical phases. Additionally, some vaccines have received Emergency Use Authorization. The article also evaluates the pros and cons of different vaccine platforms and proposes recommendations for controlling the pandemic using genetic engineering technology to design effective vaccines.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2021)

Add to Collection

Article Infectious Diseases

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): The epidemic and the challenges

Chih-Cheng Lai et al.

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2020)

Add to Collection

Article Immunology

Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine

Wanbo Tai et al.

CELLULAR & MOLECULAR IMMUNOLOGY (2020)

Add to Collection

Article Multidisciplinary Sciences

Site-specific glycan analysis of the SARS-CoV-2 spike

Yasunori Watanabe et al.

SCIENCE (2020)

Add to Collection

Article Multidisciplinary Sciences

Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor

Jun Lan et al.

NATURE (2020)

Add to Collection

Article Multidisciplinary Sciences

Structural basis of receptor recognition by SARS-CoV-2

Jian Shang et al.

NATURE (2020)

Add to Collection

Article Biochemistry & Molecular Biology

A serological assay to detect SARS-CoV-2 seroconversion in humans

Fatima Amanat et al.

NATURE MEDICINE (2020)

Add to Collection

Article Biotechnology & Applied Microbiology

SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology

Natacha S. Ogando et al.

JOURNAL OF GENERAL VIROLOGY (2020)

Add to Collection

Editorial Material Medicine, Research & Experimental

SARS-CoV-2 SPIKE PROTEIN: an optimal immunological target for vaccines

Giovanni Salvatori et al.

JOURNAL OF TRANSLATIONAL MEDICINE (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein

Lorenzo Casalino et al.

ACS CENTRAL SCIENCE (2020)

Add to Collection

Article Hematology

Heparin Inhibits Cellular Invasion by SARS-CoV-2: Structural Dependence of the Interaction of the Spike S1 Receptor-Binding Domain with Heparin

Courtney J. Mycroft-West et al.

THROMBOSIS AND HAEMOSTASIS (2020)

Add to Collection

Article Multidisciplinary Sciences

Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Claudia R. Arbeitman et al.

SCIENTIFIC REPORTS (2020)

Add to Collection

Review Immunology

SARS-CoV-2: Structure, Biology, and Structure-Based Therapeutics Development

Mei-Yue Wang et al.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2

Asif Shajahan et al.

GLYCOBIOLOGY (2020)

Add to Collection

Article Immunology

SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates

Tingting Li et al.

EMERGING MICROBES & INFECTIONS (2020)

Add to Collection

Article Biochemistry & Molecular Biology

BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra

Andras Micsonai et al.

NUCLEIC ACIDS RESEARCH (2018)

Add to Collection

Article Engineering, Chemical

Production and Purification of Recombinant Proteins from Escherichia coli

Nagesh K. Tripathi

CHEMBIOENG REVIEWS (2016)

Add to Collection

Article Multidisciplinary Sciences

Accurate secondary structure prediction and fold recognition for circular dichroism spectroscopy

Andras Micsonai et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Add to Collection

Article Biochemistry & Molecular Biology

Structure and mechanism in protein science. A guide to enzyme catalysis and protein folding, by A. Fersht. 1999. New York: Freeman. 631 pp. $67.95 (hardcover)

Robert L. Baldwin

PROTEIN SCIENCE (2010)

Add to Collection

Article Biotechnology & Applied Microbiology

Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus

Xiaofeng Ren et al.

JOURNAL OF GENERAL VIROLOGY (2006)

Add to Collection

Article Biochemistry & Molecular Biology

Monitoring protein aggregation during thermal unfolding in circular dichroism experiments

S Benjwal et al.

PROTEIN SCIENCE (2006)

Add to Collection

Article Gastroenterology & Hepatology

Receptor-binding domain of SARS-Cov spike protein: Soluble expression in E.coli, purification and functional characterization

Jing Chen et al.

WORLD JOURNAL OF GASTROENTEROLOGY (2005)

Add to Collection

© Peeref 2019-2024. All rights reserved.