Journal
VACCINES
Volume 10, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/vaccines10020163
Keywords
immunotherapy; peptide cancer vaccine; B-cell cancer vaccine; CRM197; IL-17RB; MALDI-TOF-MS; hapten density
Categories
Funding
- Academia Sinica Genomics Research Center
Ask authors/readers for more resources
This article introduces the preparation and clinical application of a cancer vaccine targeting IL-17RB. The vaccine is composed of multiple synthesized epitope peptides chemically conjugated onto CRM197, a carrier protein, and has a durable anti-cancer effect.
Immune checkpoint inhibitors such as monoclonal antibodies (mAbs) are amongst the most important breakthroughs in cancer therapeutics. However, high cost and short acting time limits its affordability and clinical application. Therefore, an economical and durable alternative is urgently needed. Previously, we identified an IL-17RB targeting mAb which intercepts IL-17B/IL-17RB signal transduction and suppresses tumorigenesis in many types of cancer. We reason that active immunity against the antigenic epitope of IL-17RB can reproduce the anti-cancer effect of mAbs with better sustainability. Here, we present a cancer vaccine composed of multiple synthesized epitope peptides chemically conjugated onto CRM197, a highly immunogenic carrier protein. Combining mass spectrometry with immunoassay, we standardized hapten density determination and optimized vaccine design. Furthermore, orthotopically transplanted syngeneic mouse tumor 4T1 showed that administration of this vaccine therapeutically mitigates primary cancer growth as well as distance metastasis. In conclusion, we demonstrate preparation, characterization and pre-clinical application of a novel peptide cancer vaccine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available