Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.810180
Keywords
inteins; synthetic biology; living therapeutics; protein engineering; split inteins
Funding
- National Key Research and Development Program of China [2018YFA0903000, 2020YFA0908100]
- Shenzhen Science and Technology Program [KQTD20180413181837372]
- National Natural Science Foundation of China [32071427]
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In this review, we discuss the recent advancements in the field of synthetic biology regarding inteins and their future directions.
Inteins are protein segments that are capable of enabling the ligation of flanking extein into a new protein, a process known as protein splicing. Since its discovery, inteins have become powerful biotechnological tools for applications such as protein engineering. In the last 10 years, the development in synthetic biology has further endowed inteins with enhanced functions and diverse utilizations. Here we review these efforts and discuss the future directions.
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